Slow Passive Diffusion of Orthophosphate between Intact Isolated Chloroplasts and Suspending Medium
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Slow Passive Diffusion of Orthophosphate between Intact Isolated Chloroplasts and Suspending Medium.
Isolated spinach chloroplasts purified by isopycnic centrifugation in density gradients of Percoll were found to be highly intact, to be devoid of extrachloroplastic contaminations, and to retain a high rate of CO(2)-dependent O(2) evolution.When suspended in a medium which avoided rupture of the envelope, intact purified chloroplasts progressively lost their phosphate content by passive diffus...
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Mass spectrometric techniques were used to trace the incorporation of 1180loxygen Into metabolites of the photorespiratory pathway. Glycolate, glycine, and serine extracted from leaves ofthe C3 plants, Spinacis okraces L., Atripex hastata, and Helianthus annuus which had been exposed to 1Oloxygen at the C02 compensation point were heavily labeled with 180. In each case one, and only one of the ...
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Linolenate has been shown [I] to inhibit photosynthesis in intact spinach chloroplasts by inhibiting the influx of orthophosphate and the efflux of 3-phosphoglyceraldehyde. The inhibitory effect of linolenate was shown to depend on the level of the internal pool of metabolites [2] as a result of experiments which on the one hand used chloroplasts from preilluminated leaves or chloroplasts which...
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Protoplasts, protoplast extracts (intact chloroplasts plus extrachloroplastic material), and chloroplasts isolated from protoplasts of wheat (Triticum aestivum) have rates of photosynthesis as measured by light-dependent O(2) evolution of about 100 to 150 micromoles of O(2) per milligram of chlorophyll per hour at 20 C and saturating bicarbonate. The assay conditions sufficient for this activit...
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Starch breakdown with rates above 10 muatom carbon per mg chlorophyll per hour has been monitored in spinach chloroplasts and compares favorably with the rates in whole leaves. Intact starch-loaded chloroplasts were prepared from protoplasts to avoid rupture during mechanical homogenization and rapid centrifugation. Particular attention was paid to the identification of all the products of star...
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ژورنال
عنوان ژورنال: Plant Physiology
سال: 1981
ISSN: 0032-0889,1532-2548
DOI: 10.1104/pp.67.3.470